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KMID : 0378019830260010096
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New Medical Journal
1983 Volume.26 No. 1 p.96 ~ p.106
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Isolation and Identification of Staphylococci from Hospital Air; Resistance in Antibiotic Sensitivity Test
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Abstract
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The patients in the hospital can be infected with nosocomial pathogens that are distributed in hospital environments. Before antimicrobial agents were abilable, staphylococci were known to be the most important nosocomial pathgens. With new available drugs and developments have changed. By the mid-1960s, gram negative bacilli were the common recognized nosocomial pathogens. Recently nosocomial infections caused by viruses have been reported. But the drug-resistant staphylococci still cause. high portions of nosocomial infections.
This investigation was performed to isolate and identify pathogenic staphylococci from air at the 25 sites in one hospital in Seoul. Drug sensitivity tests bacteriologically were also studied.
The results are summarized as follows:
1. In gram staining of isolated colonies, the number of staphylococci strains was 207(7.3%) among 2844 collected in the morning, and 182(7.2%) among 2543 collected in the afternoon. No significant differences in the number of isolates were found between morning and afternoon specimens.
2. The number of isolates slightly higher in samples collected from the outpatient treatment rooms and wards in the main building than in samples from the other sites.
3. In biochemical tests of 389 strains of isolated gram positive coda, the number of coagulase positive strains was 107 (27.5°) and coagulase negative 282(72.5%). In experiments on growth on mannitol agar containing 7.5% NaCl and mannitol fermentation tests, the number of mannitol fermentation strains was 140(36.0) and nonfermenter was 75 (19.3%) .
4. In antibiotic sensitivity tests of 125 identified strains of staphylococci, coagulase positive strains showed resistance in higher portions to penicillin (81.3%) , ampicillin (84.4%) tetracycline (75.0%) and chloramphenicol(78.1%), whereas in lower portions to gentamycin (59.4%) and kanamycin (65.6%)
5. Among coagulase negative and mannitol fermenting strains, higher portions showed resistance to penicillin (70.4%), ampicillin (68.5), tetracycline (68.5%), and chloramphenicol (72..2%), whereas lower portions showed resistance to gentamycin (45.4%) and kanamycin (49.3%).
6. The number of strains showing multiple resistance to above 6 antibiotics were 15(46.9%) among coagulase positive and mannitol non-fermenter and 35(32.4%) among coagulase negative and mannitol fermenter.
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